DESIGN, DEVELOPMENT AND CHARACTERIZATION OF BRINZOLAMIDE AND BRIMONIDINE TARTRATE NANOEMULSION FOR OPHTHALMIC DRUG DELIVERY
Bhavin R. Choradiya *, Sanjay B. Patil
Department of Pharmaceutics, SNJB’s, Shriman Sureshdada Jain College of Pharmacy, Neminagar, Chandwad, Dist. Nashik-423501, Maharashtra, India
The present research work summarizes the formulation development and characterization of brinzolamide and brimonidine tartrate ophthalmic nanoemulsion providing increased permeation and prolonged therapeutic effect for the treatment of glaucoma. Nanoemulsion was prepared using castor oil, polysorbate 80 and glycerol utilizing the spontaneous emulsification technique. Formulations were screened based on globule size, zeta potential, in vitro drug release and stability upon storage. Selected nanoemulsion formulations exhibited a low mean globule diameter (< 200 nm), enhanced permeability (> 80%) in comparison with the existing marketed suspension formulation and stability for minimum 3 months at 25 °C/40% RH and 40 °C/25% RH. Nanoemulsions were evaluated for ex vivo ocular irritation studies using the Hen's Egg Chorioallantoic Membrane test (HET-CAM) method. Tested nanoemulsion formulations were proven non-irritant in similarity like the marketed dosage form. Therefore, nanoemulsion formulation containing brinzolamide and brimonidine tartrate may serve as an improved drug delivery system providing superior therapeutic efficacy and better patient compliance for the treatment of glaucoma.
SPRINKLE FORMULATION OF AMOXICILLIN-TRIHYDRATE FOR PEDIATRIC USE
Shilpa Gupta *, Ashlesha Pandit
Department of Pharmaceutics, JSPM’s Rajarshi Shahu College of Pharmacy and Research, Tathawade, Pune, Maharashtra 411 033, India
Sprinkle formulation is gaining popularity due to applicability with soft food/liquid for paediatric use. Present study deals with paediatric formulation of amoxicillin trihydrate with honey as sweetener which was adsorbed on Neusilin US2 to get free flowing powder. Further, optimized formulation was achieved using three level, two factor factorial design. Thereafter, interaction study with soft foods like curd, milk, apple, banana, which exhibited no interaction and was found safe for paediatric use.
GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES FOR TREATMENT OF SUPERFICIAL FUNGAL INFECTIONS OF THE SKIN
Chaya G., Akash D.G.⃰
Department of Pharmaceutics, Government College of Pharmacy, No.2 P. Kalinga Rao Road, Subbaiah Circle, Bengaluru-560027, India.
Green synthesis of zinc oxide nanoparticles zinc oxide nanoparticles using neem leaf powder extract was carried out with an intent on improving topical antifungal activity. Formulations were prepared by applying Box-Behnken design using neem extract concentration (A), cetyl trimethyl ammonium bromide concentration (B) and stirring time(C) as independent variables ; particle size, practical yield and entrapment efficiency were chosen as responses respectively. zinc oxide nanoparticles were characterized by scanning electron microscopy, X-ray diffraction and EDAX techniques. The optimized zinc oxide nanoparticles were incorporated into a cream and evaluated for various parameters such as pH, viscosity, spreadability, drug content, in-vitroand in-vivo antifungal activity using Wistar albino rats. The study indicated that green synthesized zinc oxide nanoparticles exhibited greater antifungal effect as compared to chemically synthesized zinc oxide nanoparticles when incorporated into a cream.
SMEDDS FORMULATION OF GLILAZIDE WITH ENHANCED DISSOLUTION
Heta S. Vasani*, Divya D. Jain, Rajesh M. Popale, Veena P. Chaudhari, Ujwala A. Shinde.
Department of Pharmaceutics, IPA’s Bombay College of Pharmacy (Autonomous), Kalina, Santacruz (East), Mumbai, Maharashtra, India- 400098
Microemulsions are thermodynamically stable colloidal carriers that are formed spontaneously without any energy input and have generated more interest as potential drug delivery systems due to their stability and improved solubilization of hydrophobic drugs.1Self-Microemulsifying Drug Delivery systems (SMEDDS) spontaneously form o/w microemulsions on mild agitation following dilution with aqueous phases.2 Our study explores this method of solubility enhancement of a poorly water-soluble drug, gliclazide (GLZ) and evaluation of the resulting formulation. The various oil, surfactant and co surfactants were screened by their solubilizing capacity of GLZ. Optimized SMEEDs were prepared using Capryol(TM) 90, Tween 80, Gelucire® 48/16 and Transcutol® HP. The optimized formulation was evaluated for globule size, PDI, robustness to dilution, self-emulsification ability, drug content and in-vitro release. Optimized formulation exhibited globule size 11.41 nm with a PDI of 0.254. GLZ SMEDDs showed better drug release than the plain drug. Thus, GLZ SMEDDs were successfully formulated with improved solubility which was proven with reduced globule size and improved in vitro release profile.
NANOEMULGELS OF EXTRACTIVE PHYTOCONSTITUENTS OF SOYABEAN WITH PROMISING ANTI-PSORIATIC ACTIVITY
Niserga D. Sawant*, Sania K. Khan, Prachi C. Mehendale and Namita D. Desai
C. U. Shah College of Pharmacy, SNDT Women’s University,
Santacruz (W), Mumbai – 400049, Maharashtra, India. Email:
The aim of the present study was to develop and evaluate nanoemulgels of extractive phytoconstituents of seeds of Glycine max (soyabean) for management of psoriasis. Isoflavones, the most important phytoestrogen present in soyabean possess anti-inflammatory and antioxidant properties which make them a vital phytoconstituent for treating psoriasis. The ethanolic extract of soyabean (ES) was formulated into nanoemulsions using oleic acid as oil phase, Tween 20 as surfactant and polyethylene glycol 400 (PEG 400) as cosurfactant. D-optimal mixture design was applied for optimization of ES nanoemulsions and the optimized nanoemulsions were characterized for particle size, zeta potential, pH, drug content and in vitro release studies. Optimized ES nanoemulsion was further converted into ES nanoemulgel using Carbopol ULTREZ NF (1% w/w) as gelling agent. The developed nanoemulgels showed pseudo plastic behaviour with shear thinning property. In vitro release studies of ES nanoemulgels showed sustained release of active at the end of 8 hours. The results of the ex vivo permeation studies showed greater steady state flux, permeability coefficient and skin deposition in nanoemulgels as compared to in house developed conventional gels. In vivo efficacy studies using photodermatitis model in Wistar rats showed that ES nanoemulgel had comparable anti-psoriatic activity with marketed corticosteroid cream and polyherbal gel respectively. The present work thus shows the promising activity of the developed nanoemulgels of extractive phytoconstituents of soyabean when evaluated in animal models for management of psoriasis.